Dynamics of tropomyosin in muscle fibers as monitored by saturation transfer EPR of bi-functional probe.
The dynamics of four regions of tropomyosin was assessed using saturation transfer electron paramagnetic resonance in the Notebook Briefcases muscle fiber.In order to fully immobilize the spin probe on the surface of tropomyosin, a bi-functional spin label was attached to i,i+4 positions via cysteine mutagenesis.The dynamics of bi-functionally labe